The cryoprotecting effects of trehalose and dimethyl sulfoxide on alginate-chitosan encapsulated mesenchymal stem cells

RW Nurhayati, G Pratama, M Dinastri - AIP Conference Proceedings, 2022 - pubs.aip.org
… MSCs from negative effects of freezing and thawing. This study provides brief evidence of
trehalose and DMSO potencies for cryopreserving stem cell-loaded microcapsule. …

Cryostorage of mesenchymal stem cells and biomedical cell-based products

DD Linkova, YP Rubtsova, MN Egorikhina - Cells, 2022 - mdpi.com
… adhesive monolayers and to simulate a three-dimensional structure, MSCs were
cryopreserved in an encapsulated form in alginate microspheres (AMSs). AMSs are permeable to …

Preservation of human mesenchymal stromal cells in protein-supplemented alginate capsules

O Hubenia, SL Marin, N Trufanova - Problems of Cryobiology and …, 2023 - cryo.org.ua
… and overcome disadvantages of cryopreservation. Here we study the cell cycle, viability, …
different forms: monolayer, suspension, encapsulation in alginate capsules (AMS), and AMS …

Encapsulation of human limbus-derived stromal/mesenchymal stem cells for biological preservation and transportation in extreme Indian conditions for clinical use

M Damala, S Swioklo, MA Koduri, NS Mitragotri… - Scientific Reports, 2019 - nature.com
… The findings of this study suggest that alginate encapsulation is an effective method of … of
32 C-37 C also combats many of the issues associated with methods like cryopreservation 10 . …

[HTML][HTML] Alginate-Gelatin Microspheres Protect Human Mesenchymal Stem Cells During Deep Cryopreservation

S Saberianpour, R Rahbarghazi… - … Journal of Cellular …, 2020 - brieflands.com
… To the best of our knowledge, there is little knowledge related to the freezing of encapsulated
MSCs with alginate-gelatin capsules. It was demonstrated that gelatin is produced by the …

Hypothermic Preservation of Adipose-Derived Mesenchymal Stromal Cells as a Viable Solution for the Storage and Distribution of Cell Therapy Products

A Branco, AL Tiago, P Laranjeira, MC Carreira… - Bioengineering, 2022 - mdpi.com
… In this study, we aim to determine the ability of alginate encapsulation to maintain cell viability…
Alginate encapsulation also has the potential to be complementary to cryopreservation as it …

Hypothermic Storage of Human Umbilical Cord Mesenchymal Stem Cells and Their Hydrogel Constructs

X Zhang, G Zhao - Cryoletters, 2020 - ingentaconnect.com
… Another method is cryopreservation, which stores cells at … ºC, and cells will suffer from ice
injury during freezing and thawing … of PVA for alginate encapsulation can improve cell viability. …

Mesenchymal stem/stromal cells: disrupting cell therapy storage and distribution with hypothermic preservation of adipose-derived mesenchymal stromal cells

A Branco, AL Tiago, P Laranjeira, MC Carreira… - …, 2023 - comum.rcaap.pt
Encapsulation at hypothermic temperatures (eg, 2–8C) could … the ability of alginate
encapsulation to maintain cell viability, … cryopreservation in CGT logistics and supply chain, …

Development of Serum-Free Media for Cryopreservation of Hydrogel Encapsulated Cell-Based Therapeutics

Y Cui, AM Nash, B Castillo, LD Sanchez Solis… - Cellular and Molecular …, 2022 - Springer
… capsule structure, followed by viability analyses of free and alginate encapsulated cells, we
… vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) since both of these …

A novel vitrified cryopreservation approach with stem cell-laden hydrogel microcapsules

T Song, B Liu - Cryoletters, 2024 - ingentaconnect.com
… OBJECTIVE: To achieve the effective cryopreservation of stem cellladen hydrogel … -shell
alginate hydrogel encapsulation method was used to produce mesenchymal stem cell-laden …